In Vivo Toxicity Study

1. Objective: To determine the toxicity of ENERCEL® in mice.

2. Methods: Either placebo (5% ethanol) or ENERCEL® was administered to groups of 10 adolescent, male CD-I mice. The animals were injected subcutaneously with 0.35 cc of placebo or the active preparation on a daily basis for 28 consecutive days. The animals were observed daily for evidence of gross toxicity (ataxia, lethargy, respiratory distress). After 28 days, sections of harvested brains, kidneys, livers, pancreases and hearts were fixed in 10% formalin, embedded in paraffin, cut by microtome, mounted on slides, stained with hematoxylin and eosin and examined under a light microscope for histopathologic changes.

3. Results: None of the mice manifested any evidence of gross or microscopic toxicity.

Jeremiah Tilles MD
April, 1997

© 2018 Enercel | Nature Advanced - Disclaimer | Privacy Policy | Terms of Service
Top